Motores Moleculares |
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Investigadores principales
Elena Cabezón & Ignacio Arechaga (Prof. Titulares)
Otro personal:
Eric Amado, estudiante predoctoral
Yolanda GarcÃa Cazorla, predoctoral
Research interests
· Structural and biochemical approach into the molecular mechanism of RecA/AAA+ motors (ATPases Associated to a large variety of cellular Activities (i.e. DNA unwinding, protein folding, DNA transport…)
· Molecular motors working on G-quadruplex DNA structures
· From DNA transporters to protein transporters: further insights into an evolutionary scenario
· Electron microscopy of large membrane protein complexes
Funding
· Plan Nacional I+D+i (2011), Investigación Fundamental No Orientada, "Unwinding the mechanism of DNA and protein translocases"- "Mecanismos moleculares y evolutivos en motores moleculares que translocan DNA y proteínas". Ref: BFU2011-22874
Publications
Peña A, Martíin-Benito J, Valpuesta JM, Carrascosa JL, de la Cruz F, Cabezón E*, Arechaga I*. The hexameric structure of a conjugative VirB4 protein ATPase provides new insights for a functional and phylogenetic relationship with DNA translocases. J. Biol. Chem. 2012, 287(47), 39925-32. DOI:10.1074/jbc.M112.413849.
Ripoll-Rozada J, Peña A, Rivas S, Moro F, de la Cruz F, Cabezón E*,Arechaga I*. Regulation of the type IV secretion ATPase TrwD by magnesium: implications for the catalytic mechanism of the secretion ATPase superfamily. J. Biol. Chem. 2012, 287(21), 17408-14. DOI:10.1074/jbc.M112.357905.
Cabezón E*, Lanza VF, Arechaga I*. Membrane-associated nanomotors form macromolecular transport Curr. Opin. Biotechnol. 2012, 23(4), 537-44. DOI:10.1016/j.copbio.2011.11.031.
Kumar R, Ferez M, Swamy M, Arechaga I, Rejas MT, Valpuesta JM, Schamel WW, Alarcón B, van Santen HM. Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes Immunity 2011, 35(3), 375-87. DOI:10.1016/j.immuni.2011.08.010.
Peña A, Ripoll-Rozada J, Zunzunegui S, Cabezón E, de la Cruz F, Arechaga I*. Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems J. Biol. Chem. 2011, 286(19), 17376-82. DOI:10.1074/jbc.M110.208942.
Canone J, Marino D, Noel LD, Arechaga I, Pichereaux C, Rossignol M, Roby D, Rivas S*. Detection and functional characterization of a 215 amino acid N-terminal extension in the Xanthomonas type III effector XopD PLOS One 2010, 22(5), e15773. DOI:10.1371/journal.pone.0015773.
Arechaga I, Swamy M, Abia D, Schamel WW, Alarcón B, Valpuesta JM*. Structural characterization of the TCR complex by electron microscopy Int. Immunol. 2010, 22(11), 897-903. DOI:10.1093/intimm/dxq443.
Matilla I, Alfonso C, Rivas G, Bolt EL, de la Cruz F, Cabezón E*. The conjugative DNA translocase TrwB is a structure specific DNA-binding protein. J. Biol. Chem. 2010, 285(23, 17537-44. DOI:10.1074/jbc.M109.084137.
Arechaga I, Martínez-Costa OH, Ferreras C, Carrascosa JL, Aragón JJ*. Electron microscopy analysis of mammalian phosphofructokinase reveals an unusual 3-dimensional structure with significant implications for enzyme function FASEB J. 2010, 24(12), 4960-8. DOI:10.0024-4960.
Mihajlovic S, Lang S, Sut MW, Strohmaier H, Gruber CJ, Koraimann G, Cabezón E, Moncalián G, de la Cruz F,Zechner EL* . Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface. J. Bacteriol. 2009, 19(22), 6877-87. DOI:10.1128/JB.00918-09.
Arechaga I*, Peña A, Zunzunegui S, Del Carmen Fernández-Alonso M, Rivas G, de la Cruz F. ATPase activity and oligomeric state of TrwK, the VirB4 homologue of plasmid R388 Type IV secretion system. J. Bacteriol. 2008, 190(15), 5472-9. DOI:10.1128/JB.00321-08.
Tato I, Matilla I, Arechaga I, Zunzunegui S, de la Cruz F, Cabezón E*. The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA: Implications for a common assembly mechanism of DNA translocating motors. J. Biol Chem. 2007, 282(35), 25569-76. DOI:10.1074/jbc.M703464200.
Arechaga I*, Fotiadis D. Reconstitution of mitochondrial ATP synthase into lipid bilayers for structural analysis. J. Struct. Biol. 160(3, 287-94. DOI:10.1016/j.jsb.2007.09.07.
Cabezón E, de la Cruz F "TrwB: An F1-ATPase-like molecular motor involved in DNA transport during bacterial conjugation". Res. Microbiol. 2006, 157:299–305. DOI:10.1016/j.resmic.2005.12.002.
Schamel W W A, Arechaga I, Risueño RM, van Santen HM, Cabezas P, Risco C, Valpuesta JM, Alarcón B. "Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response". J Exp Med. 2005, 202:493–503. DOI:10.1084/jem.20042155.
Tato I, Zunzunegui S, de la Cruz F, Cabezón E*. "TrwB, the coupling protein involved in DNA transport during bacterial conjugation, is a DNA-dependent ATPase". Proc Natl Acad Sci USA 2005, 102:8156-61. DOI:10.1073/pnas.0503402102.
Cabezón E, Montgomery MG, Leslie AG, Walker JE*. (2003) "The structure of bovine F1-ATPase in complex with its regulatory protein IF1." Nat Struct Biol. 10(9):744-50. DOI:10.1038/nsb966.
Arechaga, I., Miroux, B., Runswick, M. J. & Walker, J. E*. (2003). "Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript". FEBS Lett 547, 97-100. DOI:10.1016/S0014-5793(03)00677-X.
Jekabsons, M. B., Echtay, K. S., Arechaga, I. & Brand, M. D. (2003). "Molecular properties of purified human uncoupling protein 2 refolded from bacterial inclusion bodies". J Bioenerg Biomembr 35, 409-18. DOI:10.1023/A:1027335713635.
Murata, T*., Arechaga, I*., Fearnley, I. M., Kakinuma, Y., Yamato, I. & Walker, J. E*. (2003). (*equal contributors) "The membrane domain of the Na+-motive V-ATPase from Enterococcus hirae contains a heptameric rotor". J Biol Chem 278, 21162-7. DOI:10.1074/jbc.M301620200
Martinez LO, Jacquet S, Esteve JP, Rolland C, Cabezon E, Champagne E, Pineau T, Georgeaud V, Walker JE, Terce F, Collet X, Perret B, Barbaras R*. (2003) "Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis." Nature 421:75-9. DOI:10.1038/nature01250.
Contact:
Dr. Elena Cabezón: cabezone@unican.es
Dr. Ignacio Arechaga: arechagai@unican.es
Phone: (+34) 942 202033 / Fax : (+34) 942 266399
Mailing address: IBBTEC. C/ Albert Einstein 22, PCTCAN, 39011 Santander
