Principal Investigator:

Dr. Matxalen Llosa, Associate Professor

 

Other personnel:

• Dra. Anabel Alperi, postdoctoral 
• Coral González Prieto, predoctoral
• Víctor Secadas, technician

Web page:

 http://grupos.unican.es/ingenet/english.htm 

 

Research lines:

Site-specific recombination and integration catalyzed by conjugative relaxases

Substrate Recruitment by Type IV Secretion systems

Molecular analysis of conjugative systems

Use of bacterial secretion systems to develop tools for the genomic modification of mammalian cells

 

Funding:

MICINN Explora BIO2010-11623, “Modificación genética de mitocondrias de mamífero mediante sistemas de secreción bacterianos”

 

 MICINN BIO2008-00133, “Desarrollo de herramientas de modificación genética de células de mamífero basadas en sistemas de secreción tipo IV bacterianos”

 

Relevant publications (last 10 years)

 Alperi A, Larrea D, Fernández-González E, Dehio C, Zechner EL, Llosa M* (2013). “A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative Type IV Secretion System”. J Bacteriol, in press.

 Larrea D, de Paz HD, Arechaga I, de la Cruz F, Llosa M* (2013). “Structural independence of conjugative coupling protein TrwB from its Type IV secretion machinery”. Plasmid 70(1):146-53.

 González-Prieto C, Agúndez L, Linden RM, Llosa M* (2013). “HUH site-specific recombinases for targeted modification of the human genome”. Trends Biotechnol 31(5):305-12.

Llosa M, Schröder G, Dehio C (2012). "New perspectives into bacterial DNA transfer to human cells. Trends in Microbiology 20(8):355-359.

Agúndez L, González-Prieto C, Machón C, Llosa M (2012). Site-Specific Integration of Foreign DNA into Minimal Bacterial and Human Target Sequences Mediated by a Conjugative Relaxase". PLoS ONE 7(1):e31047

Fernández-González E, de Paz HD, Alperi A, Agúndez L, Faustmann M, Sangari FJ, Dehio C, Llosa M (2011). “Transfer of R388 derivatives by a pathogenesis-associated type IV secretion system into both bacteria and human cells”. J Bacteriol 193 (22), 6257–6265

Agúndez L, Machón C, César CE, Rosa-Garrido M, Delgado MD, Llosa M (2011). “Nuclear targeting of a bacterial integrase which mediates site-specific recombination between bacterial and human target sequences”. Appl Env Microbiol 77(1): 201-210

de Paz HD, Larrea D, Zunzunegui S, Dehio C, de la Cruz F, Llosa M (2010). "Functional dissection of the conjugative coupling protein TrwB". J Bacteriol 192(11), 2655-2669.

Llosa M, Roy C, Dehio C (2009). Bacterial Type IV secretion systems in human disease. Mol. Microbiol. 73(2):141-151.

César CE, Llosa M (2007). TrwC-mediated site-specific recombination is controlled by host factors altering local DNA topology. J. Bacteriol. 189(24):9037-9043.

César CE, Machón C, de la Cruz F, Llosa M (2006). A new domain of conjugative relaxase TrwC responsible for efficient oriT-specific recombination on minimal target sequences. Mol. Microbiol. 62(4):984-996.

Draper O, César CE, Machón C, de la Cruz F, Llosa M (2005). Site-specific recombinase and integrase activities of a conjugative relaxase in the recipient cell. Proc. Natl. Acad. Sci. USA 102:16385-16390.

de Paz HD, Sangari FJ, Bolland S, García-Lobo JM, Dehio C, de la Cruz F, Llosa M (2005). Functional interactions between Type IV secretion systems involved in DNA transfer and virulence. Microbiology 151:3505-3516.

Llosa M, de la Cruz F (2005). Bacterial conjugation: a potential tool for genomic engineering. Res. Microbiol. 156:1-6.

Llosa M, O´Callaghan D (2004) Euroconference on the Biology of Type IV Secretion Processes: Bacterial gates into the outer world. Mol. Microbiol. 53:1-8.

Llosa M, Zunzunegui S, de la Cruz F (2003). Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes. Proc. Natl. Acad. Sci. USA 100:10460-10464.

Llosa M, Gomis-Rüth X, Coll M, de la Cruz F (2002). Bacterial conjugation. A two-step model for DNA transport. Mol. Microbiol. 45:1-8.

 

Contact:

Dr. Matxalen Llosa

Phone: (+34) 942 201957 / Fax : (+34) 942 266399

Email: llosam@unican.es

Mailing address: IBBTEC. C/ Albert Einstein 22, PCTCAN, 39011 Santander